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The samples were then centrifuged at 2500g at 4C for 15minutes and stored at 20C until analysed. Serum ALT (Archem, Istanbul, Turkey), AST (Archem), cholesterol (Archem), triglyceride (Archem), albumin (Archem), GGT (Archem), total protein (Archem), creatinine (Archem) and TAS (Real Assay Diagnostics, Istanbul, Turkey) levels were measured using commercially available kits as per manufacturer’s recommendations using a Biochemistry Auto Analyzer (Sinnowa D280, China).Serum lipid peroxidation was estimated through a previously described method (Yoshoka and others 1979) in which MDA reacts with thiobarbituric acid (TBA) to form a coloured complex at a maximum absorbance of 535nm. The TSA value was measured at 549nm using the method described by Warren (1959): sialic acid was oxidised to formyl pyruvic acid, which reacts with TBA to form a pink product (Warren 1959).
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